I buy glycerin by the gallon (usually through Amazon). Interesting side story. I started getting additional recommendations for vaping supplies after ordering the glycerin. It must be one of the carriers in addition to propylene glycol used for vaping.
I've never taken a 100% glycerin enema. The strongest mixed concentration I've ever taken is 30% and it gives a very strong peristaltic contraction reaction and produces nausea (for me) at that concentration.
The closest to a 100% glycerin enema I've taken is one where I poured a 300 ml bottom layer glycerin in a clear bag and down the hose and then topped it with 2700ml warm water and not mixed. I didn't warm the glycerin above room temperature and it is a slightly thicker and more viscous liquid under these conditions. Although there was some minimal mixing at the interface between the two liquids, two distinct layers were visible. I used a double balloon nozzle and made sure it was well-seated inside me and provided a great seal, inside and out.
When I opened the clamp, I could feel the cool glycerin enter my rectum above the balloon and fill my rectum and overflow into my Sigmoid colon. I could also watch the glycerin layer slowly descend in the bag and travel down the hose. I could feel when the warm water finally reached the end of the nozzle because I could feel the temperature change in my rectum and then spreading throughout my colon.
I cannot tell you whether the inflowing water pushed the glycerin layer forward and further into my colon (plug flow) or whether the turbulence around the nozzle tip had the water and the glycerin mix. It was probably a combination of both. I do know that once the water reached the nozzle, the flow rate increased dramatically and I stirred the water in the bag to blend the glycerin that was clinging to the bag wall with the water remaining in the bag. I'm fairly certain that the glycerin on the hose wall was swept along by the flowing liquid. The bag and hose was completely drained in five minutes (it took two minutes for the glycerin to drain into me).
From everything I had read, I expected an almost immediate reaction and was a bit surprised that I didn't have a strong reaction to push the pure glycerin or high glycerin content liquid out of me. but the whole bag was inside me and I felt something else that was really interesting. I could feel what I describe as a tingling sensation in my rectum. It wasn't a burning sensation but over the period of the next few minutes there was an increased intensity to this sensation. I also started to have the contractions in my colon that were further up and in my descending and transverse colon. They were intense but not painful as they tried to force the liquid out of my ass and the balloon nozzle was keeping that from happening.
The intensity of the stimulation of my rectum increased and it felt like the muscles of my rectum were trying to squeeze down on the inner balloon to either force it out (or pop it). There was a check valve in the line and so the enema could not flow back up the hose and towards the bag and The inner balloon was firmly seated in my rectum. I had used only enough lubricant to make sure the balloon went through my anus on the way in. I had no desire to have it be slippery enough to slide out of me. After about 5 minutes of this building intensity in my rectum, I finally decided to lower the pressure of the inner balloon. I didn't leak right away because I didn't deflate it. The tingling got more intense and I relieved a little more pressure but I remained pretty well sealed. That temporarily relieved the intensity of the tingling in my rectum. A minute later it was just or more intense than it had been and I decided to deflate the balloons and pull the nozzle out of me. Only a small amount of the enema leaked out and it was most glycerin from what I remember.
What happened next was completely unexpected. I realized that my rectum muscles were contracting and closing down my rectum. The sensation was like every nerve ending had been turned "on" with the pressure equivalent of feeling a hot enema in the rectum. I could also feel what felt like a cramp, a real cramping of my rectum. I've had rectal cramping before, associated with diarrhea, and it felt a lot like that. My reaction to the high concentration of glycerin was the stimulation/sensitizing of my rectum to the point where it "closed down." In the meantime, all this liquid was churning around further up my colon and there was very little being forced out through my rectum (a little squirt and dribble). Most of the three liters of liquid were trapped above my rectum which was closed down.
I couldn't even insert a small diameter straight nozzle to try to dilute the glycerin concentration in my rectum. Pain and pleasure. My rectum finally started to "relax" after about 20-25 minutes and the enema began to flow out of me. As soon as I could get a small nozzle inserted to allow a warm water rinse enema to get into me and start flushing out and diluting the glycerin, I did. My rectum was highly sensitized by the glycerin but eventually after about the third plain water enema, I could get one of my slightly longer and deeper small diameter nozzles in deep enough that the tip was at or above the point where the glycerin was first introduced into my rectum. By the time I completed the last rinse enema, the expulsion was clear and only slightly tinted at the end. I was well-cleaned out.
I get a similar reaction with a well-mixed glycerin enema that is in the 25-30% range. My "sweet spot" is an enema of 3.0 liters with 10% glycerin by volume and 10 grams of Ivory soap per liter. It produces a very cleansing enema with plenty of colon contractions that can lead to what feels like an anal orgasm (it originates deep inside me more to the center of my back and radiates out from there) and is different from my "normal" cum producing orgasms. It doesn't happen all the time with this combination and it is tough to hold the enema. But, when it does, it is wonderfully satisfying. The longest I've been able to hold one of these enemas and ride the waves of anal orgasms is 86 minutes when I finally went over the edge with both the deep, internal anal orgasm along with an ejaculatory orgasm. I don't know what made that enema so special. But it was wonderful.